Generator

Part:BBa_K1179072:Design

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-18)


Hef1a_eYFP-4x451


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2073
    Illegal SpeI site found at 2049
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2073
    Illegal SpeI site found at 2049
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2073
    Illegal BglII site found at 569
    Illegal BamHI site found at 1197
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2073
    Illegal SpeI site found at 2049
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2073
    Illegal SpeI site found at 2049
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal PstI site found at 1434
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There will still be low yellow fluorescence background expression in the presence of miRNA451. It is recommended that people interested in using this part, test for this small amount of background expression before performing experiments with this part.

Source

This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has been modified to fluoresce yellow and also synthesized with four miRNA451 target sites.

References